GENEWIZ NGS Sample Report
1. Sample Information
| Customer | GENEWIZ NGS |
| ngs@genewiz.com | |
| Quote Number | GW0101001 |
| Configuration | Illumina HiSeq, PE 2x150 |
| Sample | T1 |
2. Sample Sequencing Summary
Raw sequencing data are evaluated with FastQC, a tool for assessing sequencing quality. Sequencing data from each read pair are plotted in red for read 1 and blue for read 2.
2.1 Per base quality score distribution
Figure 2.1 shows the mean quality score (Q score) at each base position averaged across all reads. The x-axis represents base position in the read, and the y-axis represents the Q score. High quality samples have consistently high Q scores across the length of the read.
Figure 2.1 Base quality score distribution
2.2 Overall quality score distribution
Figure 2.2 shows the distribution of average quality score of reads. The x-axis represents the Q score, and the y-axis shows the percentage of reads has equal or higher quality score to the x-axis.
Figure 2.2 Reads quality score distribution
2.3 Per sequence GC content
Figure 2.3 shows the distribution of GC content across all reads. The x-axis represents %GC content, and the y-axis represents the proportion of all reads with a given GC content.
Figure 2.3 Reads GC content distribution
2.4 Alignment summary
Sequencing Adapters and low quality bases in raw reads were trimmed using Trimmomatic 0.38. Cleaned reads were then aligned to NA reference genome using Dragen.
Table 2.1 is the summary statistics of the aligment. Percetntages shown in the table are percentages of total cleaned read.